Effects of hypoxic preconditioning on the cytoskeleton of primarily cultured sinoatrial node cells of neonatal rat / 中国病理生理杂志

ABSTRACT

AIM: To evaluate the effect of hypoxic preconditioning (HP) on the cytoskeleton of sinoatrial node cells primarily cultured from neonatal rat. METHODS: Cells were randomized to three groups of control, hypoxia/reoxygenation ( H/R) and HP. F-actin, vinculin, ?-tubulin and desmin were studied with laser confocal microscopy after labled with different immune fluorescent agents. RESULTS: Compared with control, although both H/R and HP reduced the fluorescence intensity of these four cytoskeletal proteins remarkably (P