Effect of antisense phosphorothioate oligonucleotide on activity of telomerase for human bladder tumor cell / 临床外科杂志
Journal of Clinical Surgery
; (12)2002.
Article
ي Zh
| WPRIM
| ID: wpr-553401
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective To detect the Telomerase inhibitor-antisense phosphorothiate oligonucleotide (asONS) effect on telomerase activity of human bladder tumor cell line BIU-87.Methods BIU-87 cells and cell extracts are cultured with asONS,non-asONS and control fluid at different concentrations and times respectively.Telomerase activity was assayed by TRAP PCR-ELASA.Results AsONS can inhibit the activity of BIU-87 cells ,whereas non-asONS and control group not, and have significant difference(P
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
Zh
مجلة:
Journal of Clinical Surgery
السنة:
2002
نوع:
Article