The Suppressive Effect of TNF-? on Growth and Cell Cycle of Human Lung Adenocarcinoma Cells / 中国肿瘤生物治疗杂志

ABSTRACT

In the present investigation, the cytotoxic activity of tumor necrosis factor a was studied on human lung cancer cells in uitro. A wide range of TNF-ct concentration (from 100 to 10000 U/ml) was tested using the MTT assay. Data presented showed the suppressive effects of TNF-? on A549 dose-dependently and time-dependently; Twenty four hours exposure of A549 human cancer cells lo TNF-? shifted cells from G2 + M,S phase to GO + Gl phase as determined by analysis of isolated cell nuclei with an FACScan cell sorter. The results suggest that the mechanism of the eflect of TNF-a is to influence the cell cvcle of A549.