Effects of hyperglycemia and insulin intervention on the cerebral injury after ischemia-reperfusion in rats / 临床神经病学杂志

ABSTRACT

Objective To study the effects of hyperglycemia plasma glucose and insulin intervention on the cerebral injury after ischemia-reperfusion in rats.Methods 160 male SD rats were randomly divided into four groups:sham operation group,normal plasma glucose group,hyperglycemia group and insulin intervention group(40 rats in each group).The rats of hyperglycemia and insulin intervention groups were given STZ to induce hyperglycemia model.All the rats except for those in the sham operation group were subjected to right middle cerebral artery occlusion and then reperfusion.The reperfusion was started at 1 h,6 h,12 h and 24 h after 2 h ischemia.The neurologic functional scores,infarct volume and numbers of neuron apoptosis of the rats were analyzed with the neurological severity score(NSS),TTC staining assay and TUNEL method,respectively after the 2ats wake up.Results Compared with normal plasma glucose group,worse neurological function score,larger infarct size and more number of apoptotic neurons were found in hyperglycemia group at the same reperfusion time(all P