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A Simple Reliable Fluorescent Assay Method for Histone Acetyltransferase (HAT) Inhibitors / 生物化学与生物物理进展
Article ي Zh | WPRIM | ID: wpr-592647
المكتبة المسؤولة: WPRO
ABSTRACT
Histone acetyltransferases (HATs) are involved in the regulation of gene transcription in eukaryotic cells and their inhibitors could be a promising class of drugs due to their ability to modulate transcription and exert antiviral, anti-inflammatory as well as antioxidant effects. Nonradioactive spectrophotometric HAT assay is an alternative method to the widespread radioactive assay but suffers from drawbacks as lack of sensitivity and accuracy. A simple, non-radioactive fluorescent assay that measures the production of CoASH was established by its facile reaction with O-phthalaldehyde and 2-amino-ethanol. This method gives much higher accuracy compared to spectrophotometric assay, and allows screening of various compounds with potential HAT inhibition. The novel assay should be a valuable tool in transcriptional research and especially drug discovery.
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النص الكامل: 1 الفهرس: WPRIM اللغة: Zh مجلة: Progress in Biochemistry and Biophysics السنة: 2006 نوع: Article
النص الكامل: 1 الفهرس: WPRIM اللغة: Zh مجلة: Progress in Biochemistry and Biophysics السنة: 2006 نوع: Article