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Infection results analysis of immunofluorescence method detection of RSV / 中国医学装备
China Medical Equipment ; (12): 70-72,73, 2015.
Article ي Zh | WPRIM | ID: wpr-601932
المكتبة المسؤولة: WPRO
ABSTRACT
Objective:To analyze the infection results of The immunofluorescence detection of respiratory syncytial virus (RSV) in huazhou region from 2009 to 2010.Methods: The N gene sequence of RSV was taken as a reference to design specific primers and TaqMan fluorescent probe and to establish real-time The immunofluorescence method to detect sensitivity and specificity.21548 swab samples of children with acute respiratory infections in huazhou region from 2009 to 2010 were tested with real-time fluorescence. The immunofluorescence method.Results: The specificity and sensitivity of real-time fluorescence. The immunofluorescence method for detection of RSV infection were higher, without cross-reactivity to other respiratory viruses.10,477 cases of clinical specimens in 2009 were detected, with positive RSV infection rate of 9.73%, by real-time The immunofluorescence method detection. Positive detection rate of RSV infection had two peaks, which lied in February and December; the positive rate was 11% and 11% respectively. RSV infection rate was the highest for children under 6 years old.Conclusion: Real-time fluorescence features convenient detection, high specificity, and high sensitivity, which can be used for clinical detection of RSV infection. From 2009 to 2010, RSV infection remains a major pathogen of acute respiratory infections in children in huazhou region.
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النص الكامل: 1 الفهرس: WPRIM نوع الدراسة: Diagnostic_studies اللغة: Zh مجلة: China Medical Equipment السنة: 2015 نوع: Article
النص الكامل: 1 الفهرس: WPRIM نوع الدراسة: Diagnostic_studies اللغة: Zh مجلة: China Medical Equipment السنة: 2015 نوع: Article