Expression of cyclooxygenase-2 mRNA and identification of its splice variant in human myometrium obtained from women in labor / 华中科技大学学报(医学)(英德文版)
Article
ي En
| WPRIM
| ID: wpr-634216
المكتبة المسؤولة:
WPRO
ABSTRACT
In order to investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splicing variant of COX-2, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of COX-2. The primers were designed and synthesized according to the sequence of rat COX-2 splice variant which was discovered firstly by us. Then the splicing variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced. The results showed that the expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than that in labor women and a new band of COX-2 was obtained in myometrium from labor woman. The fragment included an unspliced intron, which pitched between exons 7 and 8. It was suggested that COX-2 gene was not only expressed highly in human myometrium from woman in labor, but also produced splicing variant by alternative splicing.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Labor Onset
/
RNA, Messenger
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Molecular Sequence Data
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Base Sequence
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Amino Acid Sequence
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Sequence Analysis
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Cyclooxygenase 2
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Myometrium
نوع الدراسة:
Diagnostic_studies
اللغة:
En
مجلة:
Journal of Huazhong University of Science and Technology (Medical Sciences)
السنة:
2005
نوع:
Article