Effect of regulation in human umbilical vein endothelia cells treated by microRNA-132 / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
; (12): 1820-1823, 2016.
Article
ي Zh
| WPRIM
| ID: wpr-637948
المكتبة المسؤولة:
WPRO
ABSTRACT
AIM:To evaluate the regulatory effect of microRNA-132 ( miR - 132 ) in human umbilical vein endothelial cell ( HUVEC) . ●METHODS: ln vitro cultured human umbilical vein endothelia cells in hypoxic environment for 6h, then maintained under normal oxygen condition for 3h, 6h, 12h, 24h. miR-132 and peroxisome-proliferator-activated receptor-γ coactivator-1α ( PGC-1α) expression was detected by quantitative Real - time polymerase chain reaction and Western blot analysis. Human umbilical vein endothelial cells transfected miR-132 mimic and miR-132 inhibitor( anti-miR-132 ) were measured by quantitative Real-time polymerase chain reaction and Western blot. ●RESULTS: miR - 132 and PGC - 1α expression was significantly ( P ●CONCLUSION:miR-132 level is highly expressed in the HUVEC under hypoxia and may be an effect of regulation for PGC-1α.
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
Zh
مجلة:
International Eye Science
السنة:
2016
نوع:
Article