Preliminary evaluation of biodegradable implant for intraocular sustained-release of cefuroxime in rabbits / 上海第二医科大学学报

ABSTRACT

Objective To evaluate the anti-infection effects of biodegradable implant for sustained release of cefuroxime,which can be implanted into the anterior chamber in rabbits. Methods The implants for sustained release of cefuroxime were prepared with appropriate mixtures of cefuroxime axetil(CAE),poly(lactic-glycolic) acid(PLGA) and polyvinyl pyrrolidone by solvent evaporation method.Fifty rabbits were selected and divided into experiment group(n=35) and control group(n=15).The concentrations of cefuroxime in aqueous humor 0.5,1,2,6 and 24 h after subconjunctival injection of 125 mg cefuroxime were measured in control group,and those in aqueous humor and plasma 1,2,3,5,7,14 and 28 d after implantation into anterior chamber were detected in experiment group.Besides,for experiment group,the intraocular pressure before and after the implantation were obtained;the inflammation of anterior chamber was routinely observed by slit lamp;and cornea tissues were harvested for scanning electron microscopy and light microscopy. ResultsThe concentration of cefuroxime in aqueous humor in control group reached the highest at 0.5 h after injection of cefuroxime(47 736.18 ng/mL),while that was extremely low 24 h later(10.92 ng/mL).The concentrations of cefuroxime in aqueous humor were higher than 500 ng/mL within 7 d after implantation,and that was very low at d28(59.20?39.05 ng/mL).And the plasma concentrations of cefuroxime had been at lower levels ever since the implantation(