Transcription and translation of Dickkopf-1 in endometrium of pregnant mice during the peri-implantation period / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 625-7, 638, 2004.
Article
ي En
| WPRIM
| ID: wpr-640985
المكتبة المسؤولة:
WPRO
ABSTRACT
To study the expression of Dickkopf-1 (DKK-1) in endometrium of pregnant mice during the peri-implantation period and the role of DKK-1 during the embryo implantation in mice. Immunohistochemical technique was employed to determine the location of DKK-1 protein in endometrium, and semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was utilized to determine the levels of DKK-1 mRNA. Our results showed that the expressions of DKK1 mRNA and protein were higher in experimental groups than in control group (P<0.01) and it increased significantly on day 3 and reached its peak on day 4, and then decreased gradually on day 5-7. The levels of DKK-1 mRNA and protein on day 4 was significantly higher than those of other groups (P<0.01). It is concluded that DKK-1 probably plays an important role in signal transudation of embryo implantation and its high expression indicates the opening of implantation window.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Embryo Implantation
/
Transcription, Genetic
/
Protein Biosynthesis
/
RNA, Messenger
/
Intercellular Signaling Peptides and Proteins
/
Endometrium
اللغة:
En
مجلة:
Journal of Huazhong University of Science and Technology (Medical Sciences)
السنة:
2004
نوع:
Article