Comparison of three serological methods in detection of Yersina pestis F1 antibody / 中国地方病学杂志

ABSTRACT

ObjectiveTo compare the effect of three serological methods for detection of Yersina pestis F1 antibody.MethodsF1 antibody of Yersinapestis was detected with the methods of enzyme linked immunosorbent assay(EL1SA),indirect hemagglutination assay(IHA) and gold-immunochromatography assay (GICA),respectively.ResultsThe highest antibody titer was 1 ∶ 5120 by ELISA and 1 ∶ 640 by IHA.Meanwhile,the highest antibody titer of GICA was 1∶ 1280.ConclusionsEL1SA is the most sensitive method in detection of Yersina pestis F1 antibody.The sensitivity of GICA is low and that of IHA is the lowest of three serological methods.