Comparative Study of BMP-2 Alone and Combined with VEGF Carried by Hydrogel for Maxillary Alveolar Bone Regeneration
Tissue Engineering and Regenerative Medicine
; (6): 171-181, 2016.
Article
ي En
| WPRIM
| ID: wpr-646891
المكتبة المسؤولة:
WPRO
ABSTRACT
The effect of vascular endothelial growth factor (VEGF) combined with bone morphogenetic protein-2 (BMP-2) for bone regeneration is still controversial as to whether or not VEGF has a synergistic or additive effect. This study attempted to evaluate the synergistic effect of VEGF and BMP-2 compared to BMP-2 alone for maxillary alveolar bone regeneration using collagen sponge/hydrogel complex sheets in a canine model. After mixing BMP-2 and VEGF with a hyaluronic acid-based hydrogel (HAH), the collagen sponge/hydrogel complex was transplanted into maxillary alveolar bone defects (n=14) after the extraction of canine upper first molars on both sides. Bone regeneration was evaluated in three groups (control group without growth factors, experimental groups I and II with BMP-2 alone and BMP-2 and VEGF, respectively) using micro-computed tomography and histological staining. The total amount of new bone formations and bone mineral density were significantly higher in the group with BMP-2 only and the group with BMP-2 combined with VEGF than it in the control group. The area with positive staining of von Willebrand factor bone defect was significantly greater in the group with BMP-2 only and with dual growth factors than the control. BMP-2 released from the HAH promoted new bone formation. However, the combination of BMP-2 and VEGF did not show a synergistic or additive effect on bone regeneration at canine maxillary alveolar bone defects.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Osteogenesis
/
Bone Regeneration
/
Von Willebrand Factor
/
Bone Density
/
Collagen
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Hydrogels
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Intercellular Signaling Peptides and Proteins
/
Vascular Endothelial Growth Factor A
/
Molar
نوع الدراسة:
Prognostic_studies
اللغة:
En
مجلة:
Tissue Engineering and Regenerative Medicine
السنة:
2016
نوع:
Article