Simultaneous Determination of Three Components in Xiaoji Huachong Powder by UPLC / 中国药师

ABSTRACT

Objective:To establish a method for simultaneously determining 3 components ( hesperidin, magnolol and honokiol) in Xiaoji Huachong powder by UPLC. Methods: The experiments were performed on a Thermo Accucore C18 column ( 150 mm × 4. 6 mm, 2. 6 μm). The mobile phase was methanol-0. 5% glacial acetic acid in water with gradient elution, the flow rate was 0. 7 ml ·min-1 , the column temperature was maintained at 30 ℃,the detection wavelength was set at 283 nm and the injection volume was 2μl. Results:The 3 constituents showed a good linear relationship within the range of 4. 707-28. 242 μg·ml-1(r=0. 9994) for hes-peridin, 5. 085-30. 510 μg·ml-1(r=0. 9997) for magnolol and 10. 651-63. 908 μg·ml-1(r=0. 9995) for honokiol. The average recovery was 101. 2%,104. 8% and 99. 4% with the RSDs of 1. 11%,1. 68% and 1. 49% (n=6), respectively. Conclusion: The experiment provides a simple and effective method that can be used to evaluate the quality of Xiaoji Huachong powder.