Quality Standard for Mongolia Drug Liangxue Shiwei San / 中国药师
China Pharmacist
; (12): 756-758, 2015.
Article
ي Zh
| WPRIM
| ID: wpr-669779
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective:
To establish the identification method for Aucklandia Lappa and Picrorhiza kurrooa Royle ex Benth and the content determination of hydroxysaffor yellow A in Liangxue Shiwei San.Methods:
The identification was carried out by TLC. The con-tent of hydroxysaffor yellow A was determined by HPLC. The column was Kormasil C18 (250 mm × 4. 6 mm, 5 μm) and the flow rate was 1. 0 ml·min-1. The mobile phase was methanol-0. 5% acetic acid (30∶70). The detection wavelength was 403 nm and the col-umn temperature was 25℃,and the sample size was 10 μl.Results:
The TLC spots were clear with high resolution without interference from the negative sample. A good linearity of hydroxysaffor yellow A was within the range of 1.212-48.480μg·ml-1(r=0.999 8). The average recovery was 98. 13%(RSD=1. 6%,n=6).Conclusion:
The established TLC and HPLC methods are simple and accu-rate with good reproducibility, which can be used in the quality control of Liangxue Shiwei San.
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
Zh
مجلة:
China Pharmacist
السنة:
2015
نوع:
Article