A High-throughput and Quantitative Assay Based on Fluorescence Intensity for Detection of Apoptosis / 中国生物工程杂志
China Biotechnology
; (12)2006.
Article
ي Zh
| WPRIM
| ID: wpr-685016
المكتبة المسؤولة:
WPRO
ABSTRACT
Based on the different permeability of DNA-intercalant dyes YO-PRO-1(YP) and propidium iodide (PI) to the membrane of viable, apoptotic and necrotic cells, cell samples were stained with 4?mol/L YP and 4?g/ml PI for 10 min, and the fluorescence intensity of both YP and PI were measured by fluorometer at Ex/Em wavelength of 485/538nm and 530/590nm, respectively. The correlation between YP fluorescence intensity and the apoptotic cell number was confirmed by fluorescence microscope and linear regression(r=0.999,P
النص الكامل:
1
الفهرس:
WPRIM
نوع الدراسة:
Diagnostic_studies
اللغة:
Zh
مجلة:
China Biotechnology
السنة:
2006
نوع:
Article