Immunohistochemical evaluation of the goat forestomach during prenatal development
Journal of Veterinary Science
; : 35-43, 2014.
Article
ي En
| WPRIM
| ID: wpr-69672
المكتبة المسؤولة:
WPRO
ABSTRACT
Here we report the detection and distribution of synaptophysin (SPY), non-neuronal enolase (NNE), glial fibrillary acidic protein (GFAP), vimentin (VIM), neuropeptide Y (NPY), and vasoactive intestinal peptide (VIP) expression in the goat forestomach during prenatal development. A total of 140 embryos and fetuses were examined to evaluate protein expression from the first stage of prenatal life until birth. In all cases, SPY immunoreactivity was detected at 53 days gestation in the lamina propria-submucosa, tunica muscularis, serosa, and myenteric plexuses. Immunoreactivity to NNE was observed at 64 days gestation in the same locations as well as the epithelial layer. Glial cells were found at 64 days as indicated by signals corresponding to GFAP and VIM at 39 days. Positive staining for NPY and VIP was observed at 113, 75, and 95 days in the rumen, reticulum, and omasum, respectively, in the lamina propria-submucosa, tunica muscularis, and myenteric plexuses of each of these gastric compartments. These findings indicate possible preparation of the fetal goat forestomach for postnatal function. Compared to other ruminant species, neuroendocrine cells, glial cells and peptidergic innervations markers were detected earlier compared to sheep but at around the same stage as in deer.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Rumen
/
Goats
/
Immunohistochemistry
/
Biomarkers
/
Proteins
/
Neuroglia
/
Gene Expression Regulation, Developmental
/
Embryo, Mammalian
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Endocrine Cells
/
Neuroendocrine Cells
المحددات:
Animals
اللغة:
En
مجلة:
Journal of Veterinary Science
السنة:
2014
نوع:
Article