Nested-PCR and a New ELISA-Based NovaLisa Test Kit for Malaria Diagnosis in an Endemic Area of Thailand
The Korean Journal of Parasitology
; : 377-381, 2014.
Article
ي En
| WPRIM
| ID: wpr-70515
المكتبة المسؤولة:
WPRO
ABSTRACT
Microscopy is considered as the gold standard for malaria diagnosis although its wide application is limited by the requirement of highly experienced microscopists. PCR and serological tests provide efficient diagnostic performance and have been applied for malaria diagnosis and research. The aim of this study was to investigate the diagnostic performance of nested PCR and a recently developed an ELISA-based new rapid diagnosis test (RDT), NovaLisa test kit, for diagnosis of malaria infection, using microscopic method as the gold standard. The performance of nested-PCR as a malaria diagnostic tool is excellent with respect to its high accuracy, sensitivity, specificity, and ability to discriminate Plasmodium species. The sensitivity and specificity of nested-PCR compared with the microscopic method for detection of Plasmodium falciparum, Plasmodium vivax, and P. falciparum/P. vivax mixed infection were 71.4 vs 100%, 100 vs 98.7%, and 100 vs 95.0%, respectively. The sensitivity and specificity of the ELISA-based NovaLisa test kit compared with the microscopic method for detection of Plasmodium genus were 89.0 vs 91.6%, respectively. NovaLisa test kit provided comparable diagnostic performance. Its relatively low cost, simplicity, and rapidity enables large scale field application.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Thailand
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
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Polymerase Chain Reaction
/
Sensitivity and Specificity
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Malaria, Vivax
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Malaria, Falciparum
/
Endemic Diseases
/
Molecular Diagnostic Techniques
/
Coinfection
نوع الدراسة:
Diagnostic_studies
المحددات:
Adolescent
/
Adult
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Aged
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Aged80
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Female
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Humans
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Male
البلد/الأقليم حسب الموضوع:
Asia
اللغة:
En
مجلة:
The Korean Journal of Parasitology
السنة:
2014
نوع:
Article