Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells
The Korean Journal of Physiology and Pharmacology
; : 427-433, 2010.
Article
ي En
| WPRIM
| ID: wpr-727387
المكتبة المسؤولة:
WPRO
ABSTRACT
This study was conducted to investigate the effects of extremely low frequency electromagnetic fields (EMF) on signal pathway in plasma membrane of cultured cells (RAW 264.7 cells and RBL 2H3 cells), by measuring the activity of phospholipase A2 (PLA2), phospholipase C (PLC) and phospholipase D (PLD). The cells were exposed to the EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. The basal and 0.5 microM melittin-induced arachidonic acid release was not affected by EMF in both cells. In cell-free PLA2 assay, we failed to observe the change of cPLA2 and sPLA2 activity. Also both PLC and PLD activities did not show any change in the two cell lines exposed to EMF. This study suggests that the exposure condition of EMF (60 Hz, 0.1 or 1 mT) which is 2.4 fold higher than the limit of occupational exposure does not induce phospholipases-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Type C Phospholipases
/
Phospholipase D
/
Phospholipases
/
Pyridoxal
/
Signal Transduction
/
Cell Line
/
Cell Membrane
/
Cells, Cultured
/
Occupational Exposure
/
Arachidonic Acid
اللغة:
En
مجلة:
The Korean Journal of Physiology and Pharmacology
السنة:
2010
نوع:
Article