Curcumin protects against the intestinal ischemia-reperfusion injury: involvement of the tight junction protein ZO-1 and TNF-alpha related mechanism
The Korean Journal of Physiology and Pharmacology
; : 147-152, 2016.
Article
ي En
| WPRIM
| ID: wpr-728541
المكتبة المسؤولة:
WPRO
ABSTRACT
Present study aimed to investigate the eff ect of curcumin-pretreatment on intestinal I/R injury and on intestinal mucosa barrier. Thirty Wistar rats were randomly divided into: sham, I/R, and curcumin groups (n=10). Animals in curcumin group were pretreated with curcumin by gastric gavage (200 mg/kg) for 2 days before I/R. Small intestine tissues were prepared for Haematoxylin & Eosin (H&E) staining. Serum diamine oxidase (DAO) and tumor necrosis factor (TNF)-alpha levels were measured. Expression of intestinal TNF-alpha and tight junction protein (ZO-1) proteins was detected by Western blot and/or immunohistochemistry. Serum DAO level and serum and intestinal TNF-alpha leves were signifi cantly increased after I/R, and the values were markedly reduced by curcumin pretreatment although still higher than that of sham group (p<0.05 or p<0.001). H&E staining showed the significant injury to intestinal mucosa following I/R, and curcumin pretreatment signifi cantly improved the histological structure of intestinal mucosa. I/R insult also induced significantly down-regulated expression of ZO-1, and the eff ect was dramatically attenuated by curcumin-pretreatment. Curcumin may protect the intestine from I/R injury through restoration of the epithelial structure, promotion of the recovery of intestinal permeability, as well as enhancement of ZO-1 protein expression, and this eff ect may be partly attributed to the TNF-alpha related pathway.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Permeability
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Immunohistochemistry
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Reperfusion Injury
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Blotting, Western
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Tumor Necrosis Factor-alpha
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Amine Oxidase (Copper-Containing)
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Rats, Wistar
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Eosine Yellowish-(YS)
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Tight Junctions
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Curcumin
المحددات:
Animals
اللغة:
En
مجلة:
The Korean Journal of Physiology and Pharmacology
السنة:
2016
نوع:
Article