Serum Levels of Eosinophil-Derived Neurotoxin: A Biomarker for Asthma Severity in Adult Asthmatics
Allergy, Asthma & Immunology Research
; : 394-405, 2019.
Article
ي En
| WPRIM
| ID: wpr-739408
المكتبة المسؤولة:
WPRO
ABSTRACT
PURPOSE: Eosinophilic inflammation is a key component of severe asthma (SA). However, there has been no reliable serum biomarker for the eosinophilic inflammation of SA. We hypothesized that serum eosinophil-derived neurotoxin (EDN) could predict the eosinophilic inflammation of SA in adult asthmatics. METHODS: Severe asthmatics (n = 235), nonsevere asthmatics (n = 898), and healthy controls (n = 125) were enrolled from Ajou University Hospital, South Korea. The serum levels of EDN and periostin were measured by enzyme-linked immunosorbent assay and compared between severe and nonsevere asthmatics. Their associations with total eosinophil count (TEC) and clinical parameters were evaluated; clinical validation of the K-EDN kit for the measurement of serum EDN was evaluated. RESULTS: Severe asthmatics were older and had longer disease duration with significantly lower levels of forced expiratory volume in 1 second and methacholine PC20 than nonsevere asthmatics. Significant differences were found in TEC or sputum eosinophil count (%) between the groups. The serum levels of EDN and periostin were significantly higher in severe asthmatics than in nonsevere asthmatics and in healthy controls (all P < 0.05). Although significant correlations were found between serum EDN levels measured by the 2 kits (ρ = 0.545, P < 0.0001), higher correlation coefficients between serum EDN levels measured by the K-EDN kit and TEC were higher (ρ = 0.358, P < 0.0001) than those between serum EDN levels measured by the MBL kit and TEC (ρ = 0.319, P < 0.0001) or serum periostin level (ρ = 0.222, P < 0.0001). Multivariate regression analysis demonstrated that serum EDN levels measured by the K-EDN kit predicted the phenotype of SA (P = 0.003), while 2 other biomarkers did not. CONCLUSIONS: The serum EDN level may be a useful biomarker for assessing asthma severity in adult asthmatics.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Phenotype
/
Asthma
/
Sputum
/
Enzyme-Linked Immunosorbent Assay
/
Biomarkers
/
Forced Expiratory Volume
/
Methacholine Chloride
/
Eosinophil-Derived Neurotoxin
/
Eosinophils
/
Inflammation
نوع الدراسة:
Prognostic_studies
المحددات:
Adult
/
Humans
البلد/الأقليم حسب الموضوع:
Asia
اللغة:
En
مجلة:
Allergy, Asthma & Immunology Research
السنة:
2019
نوع:
Article