Simultaneous determination of four components in Sanhuang tablets by RP-HPLC / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
; (6): 279-283, 2019.
Article
ي Zh
| WPRIM
| ID: wpr-743139
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective To establish a RP-HPLC method for simultaneously determining berberine hydrochloride, baicalin, emodin and chrysophanol in Sanhuang tablets. Methods The analysis was performed on an Thermo Hypersil GOLD aQ column (250 mm×4.6 mm, 5.0 μm) with gradient elution of methanol and 0.1%orthophosphoric acid at the flow rate of 1.0 ml/min. The column temperature was 30 , and the detection wave℃length was 254 nm. Results The peaks of berberine hydrochloride, baicalin, emodin and chrysophanol were successfully separated from each other. The liner ranges of calibration curves were 0-0.6348 μg (r=0.9999), 0-1.0172 μg (r=0.9999), 0-0.8024 μg (r=0.9999), 0-0.9672 μg (r=0.9999). The average recoveries (n=9) were 101.03%, 99.81%, 99.35%, 99.54%, respectively. The limits of quantification (LOQs) were 0.0363, 0.0210, 0.0497, 0.0793 mg/g, respectively. The sample solution was stable within 24 h, the RSD (n=5) were 0.48%, 0.56%, 0.68%, 0.49%, respectively. The robustness was investigated by varying the conditions of column temperature (± 1 ℃) and flow rate (± 0.1 ml/min), with the RSD s 0.57% and 0.42%, 0.44% and 0.65%, 0.74% and 0.29%, 0.46% and 0.56%, respectively. The contents of berberine hydrochloride, baicalin, emodin and chrysophanol in twelve samples were 4.6303-5.5866, 14.5514-18.8189, 0.1711-2.7984, 0.5299-2.9254 mg/tablet, respectively. Conclusion The establish method is validated to be suitable for quality control of Sanhuang tablets.
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
Zh
مجلة:
International Journal of Traditional Chinese Medicine
السنة:
2019
نوع:
Article