Effect of Amniotic Membrane Extract on Cultured Human Nasal Mucosa Fibroblasts
Journal of the Korean Ophthalmological Society
; : 1939-1944, 2015.
Article
ي Ko
| WPRIM
| ID: wpr-74924
المكتبة المسؤولة:
WPRO
ABSTRACT
PURPOSE:
To investigate the effects of amniotic membrane extract (AME) on the survival of cultured human nasal mucosa fibroblasts.METHODS:
Primary cultured human nasal mucosa fibroblasts were exposed to 0, 10, 20, or 30 microg/mL AME for 3 days. The survival of the human nasal mucosa fibroblasts was measured using the MTT assay and apoptosis was evaluated with flow cytometry using annexin-V/propidium iodide double staining.RESULTS:
AME decreased significantly in fibroblast proliferation after exposure to 10 microg/mL (p = 0.000), and caused significant apoptosis of the fibroblasts after exposure to 10 microg/mL (p = 0.024).CONCLUSIONS:
AME decreased fibroblast proliferation in vitro at least through induction of apoptosis. Therefore, adjuvant use of AME during endonasal dacryocystorhinostomy may improve clinical outcomes.Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Dacryocystorhinostomy
/
Apoptosis
/
Fibroblasts
/
Flow Cytometry
/
Amnion
/
Nasal Mucosa
المحددات:
Humans
اللغة:
Ko
مجلة:
Journal of the Korean Ophthalmological Society
السنة:
2015
نوع:
Article