Porphyromonas gingivalis accelerates atherosclerosis through oxidation of high-density lipoprotein
Journal of Periodontal & Implant Science
; : 60-68, 2018.
Article
ي En
| WPRIM
| ID: wpr-766044
المكتبة المسؤولة:
WPRO
ABSTRACT
PURPOSE: The aim of this study was to evaluate the ability of Porphyromonas gingivalis (P. gingivalis) to induce oxidation of high-density lipoprotein (HDL) and to determine whether the oxidized HDL induced by P. gingivalis exhibited altered antiatherogenic function or became proatherogenic. METHODS: P. gingivalis and THP-1 monocytes were cultured, and the extent of HDL oxidation induced by P. gingivalis was evaluated by a thiobarbituric acid-reactive substances (TBARS) assay. To evaluate the altered antiatherogenic and proatherogenic properties of P. gingivalis-treated HDL, lipid oxidation was quantified by the TBARS assay, and tumor necrosis factor alpha (TNF-α) levels and the gelatinolytic activity of matrix metalloproteinase (MMP)-9 were also measured. After incubating macrophages with HDL and P. gingivalis, Oil Red O staining was performed to examine foam cells. RESULTS: P. gingivalis induced HDL oxidation. The HDL treated by P. gingivalis did not reduce lipid oxidation and may have enhanced the formation of MMP-9 and TNF-α. P. gingivalis-treated macrophages exhibited more lipid aggregates than untreated macrophages. CONCLUSIONS: P. gingivalis induced HDL oxidation, impairing the atheroprotective function of HDL and making it proatherogenic by eliciting a proinflammatory response through its interaction with monocytes/macrophages.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Periodontitis
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Monocytes
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Cardiovascular Diseases
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Cholesterol
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Tumor Necrosis Factor-alpha
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Thiobarbituric Acid Reactive Substances
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Porphyromonas
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Porphyromonas gingivalis
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Atherosclerosis
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Foam Cells
اللغة:
En
مجلة:
Journal of Periodontal & Implant Science
السنة:
2018
نوع:
Article