Effect of Quercetin on the Cell Cycle and Adhesion Molecules of NOD/SCID Mice with Acute B Lymphocytic Leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1616-1620, 2018.
Article
ي Zh
| WPRIM
| ID: wpr-773047
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of Quercetin on cell cycle and adhesive molecules of NOD.SCID mice with acule B lymphocytic leuaemia(B-ALL).@*METHODS@#5×10 Nalm-6(B-ALL cell line) cells were injected into the tail vein of 48 NOD/SCID mice to establish the NOD/SCID mice with B-ALL. After 15 day, the NOD/SCID mice with B-ALL were randomly divided into 3 groups: salive group as control (injection with saline of 0.2 ml/mouse), cyclophos-phamid group (injection with cyclophosphamide of 100µg/kg) and quercetin group(injection with quercetin of 3 mg/kg). After treatment for 21 d, the perecntage of Nalm-6 cells in G1, G2, M and S phases was detected by flow cytonetry; the B lymphocytes Nalm-6 cells, neutrophils and WBC in while blood were counted before and after treatment; the expression of intercellalar. Adhesion molecole-1(FCAIU-1), vascular cell adhesion molecule-1(VCAM-1) and P-selectin was detected by double autibody soundwich method.@*RESULTS@#Compared with level before treatment, the expression of ICAM-1, VCAM-1 and P-selectin decreased after treatment with guercetin, The hemogram showed that the peripheral blood nentrophil level obviously increased, while the levels of B lymphocytes, Nalm-6 cells and WBC count decreased obviously after treatment with guercetin. The cell proliferatim rario in G0/G1 phase decreased, yet the cell proliferation ratio in S and G2/M phases increased after treatment with guercetin.@*CONCLUSION@#The guercetin can decrease the intercellular adhesion through inhibition of ICAM-1 expression, and arrests Nalm-6 cells in S and G2/M phases. The guercetin has obviously inhibitory effect on B-ALL cells.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Quercetin
/
Leukemia, B-Cell
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Cell Adhesion Molecules
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Cell Adhesion
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Mice, SCID
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Mice, Inbred NOD
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Intercellular Adhesion Molecule-1
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Vascular Cell Adhesion Molecule-1
المحددات:
Animals
اللغة:
Zh
مجلة:
Journal of Experimental Hematology
السنة:
2018
نوع:
Article