NANOG Alleviates the Damage of Human Hair Follicle Mesenchymal Stem Cells Caused by H2O2 through Activation of AKT Pathway / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 272-280, 2019.
Article
ي En
| WPRIM
| ID: wpr-773396
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE@#To explore the protective effect of NANOG against hydrogen peroxide (H2O2) -induced cell damage in the human hair follicle mesenchymal stem cells (hHF-MSCs).@*METHODS@#NANOG was expressed from a lentiviral vector, pLVX-IRES-ZsGreen. NANOG hHF-MSCs and vector hHF-MSCs were treated with 400 μmol/L hydrogen peroxide (H2O2) for 2 h, the cell survival rate, cell morphology, ROS production, apoptosis and expression of AKT, ERK, and p21 were determined and compared.@*RESULTS@#Our results showed that NANOG could activate AKT and upregulate the expression of p-AKT, but not p-ERK. When treated with 400 μmol/L H2O2, NANOG hHF-MSCs showed higher cell survival rate, lower ROS production and apoptosis, higher expression of p-AKT, higher ratio of p-AKT/AKT.@*CONCLUSION@#Our results suggest that NANOG could protect hHF-MSCs against cell damage caused by H2O2 through activating AKT signaling pathway.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
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Signal Transduction
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Cell Survival
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Lentivirus
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Oxidative Stress
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Hair Follicle
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Phosphatidylinositol 3-Kinases
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Cell Biology
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Drug Evaluation, Preclinical
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Proto-Oncogene Proteins c-akt
المحددات:
Humans
اللغة:
En
مجلة:
Biomedical and Environmental Sciences
السنة:
2019
نوع:
Article