Rapamycin treatment starting at 24 h after cerebral ischemia/reperfusion exhibits protective effect on brain injury in rats / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 443-449, 2018.
Article
ي Zh
| WPRIM
| ID: wpr-775295
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE@#To investigate whether rapamycin treatment starting at 24 h after cerebral ischemia/reperfusion(I/R) has protective effect on brain injury in rats.@*METHODS@#The rat I/R model was established by middle cerebral artery occlusion according to Longa's method. A total of 104 Sprague Dawley rats were randomly divided into sham group, model group, and rapamycin-treated groups (6 h or 24 h after modeling). Neurological function was assessed with neurological severity score (NSS). Triphenyl tetrazolium chloride (TTC) staining and Fluoro-Jade B (FJB) staining were used to examine the infarct volume and neuronal apoptosis, respectively. The expression of p-S6 protein in mTOR signaling pathway was detected by Western blot analysis.@*RESULTS@#Compared with sham group, NSS of the model group was significantly increased and TTC staining indicated obvious infarct area (all 0.05).@*CONCLUSIONS@#Rapamycin treatment starting at 24 h after I/R exhibits protective effect on brain injury in rats.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Reperfusion Injury
/
Random Allocation
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Brain Ischemia
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Treatment Outcome
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Rats, Sprague-Dawley
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Sirolimus
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Infarction, Middle Cerebral Artery
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Therapeutic Uses
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Drug Therapy
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Immunosuppressive Agents
نوع الدراسة:
Prognostic_studies
المحددات:
Animals
اللغة:
Zh
مجلة:
Journal of Zhejiang University. Medical sciences
السنة:
2018
نوع:
Article