Establishment of a quantitative real-time PCR for rapid detection of Panax quinquefolius / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4050-4054, 2018.
Article
ي Zh
| WPRIM
| ID: wpr-775380
المكتبة المسؤولة:
WPRO
ABSTRACT
In this study, the specific primers and probes of Panax quinquefolius were designed for a quantitative real-time PCR, and the rapid identification method of P. quinquefolius was established by optimizing conditions. The method was used to validate 43 samples of the traditional Chinese medicine,and the results showed that 22 samples of P. quinquefolius were identified accurately. The limit of detection of the method can be reach to 1×10⁻⁴ ng. The method is accurate, fast, sensitive and specifically.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Reference Standards
/
Drugs, Chinese Herbal
/
DNA Probes
/
DNA Primers
/
Real-Time Polymerase Chain Reaction
/
Genetics
/
Panax
نوع الدراسة:
Diagnostic_studies
اللغة:
Zh
مجلة:
China Journal of Chinese Materia Medica
السنة:
2018
نوع:
Article