Comparison of gel-aided sample preparation (GASP) and two in-solution digestion workflows for proteomic analysis using Saccharomyces cerevisiae lysate
Malaysian Journal of Microbiology
; : 579-584, 2018.
Article
ي En
| WPRIM
| ID: wpr-780452
المكتبة المسؤولة:
WPRO
ABSTRACT
Abstracts@#Using Saccharomyces cerevisiae lysate, two in-solution trypsin digestions (chloroform-methanol-water precipitation and RapiGest) were compared to the recently reported gel-aided sample preparation (GASP) workflow. Our proteomic results showed that GASP afforded the highest number of overall protein identifications and peptide spectrum matches without systematic bias towards peptide or protein size.
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
En
مجلة:
Malaysian Journal of Microbiology
السنة:
2018
نوع:
Article