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The p90 ribosomal S6 kinase 2 specifically affects mitotic progression by regulating the basal level, distribution and stability of mitotic spindles
Article ي En | WPRIM | ID: wpr-78630
المكتبة المسؤولة: WPRO
ABSTRACT
RSK2, also known as RPS6KA3 (ribosomal protein S6 kinase, 90 kDa, polypeptide 3), is a downstream kinase of the mitogen-activated protein kinase (MAPK) pathway, which is important in regulating survival, transcription, growth and proliferation. However, its biological role in mitotic progression is not well understood. In this study, we examined the potential involvement of RSK2 in the regulation of mitotic progression. Interestingly, depletion of RSK2, but not RSK1, caused the accumulation of mitotic cells. Time-lapse analysis revealed that mitotic duration, particularly the duration for metaphase-to-anaphase transition was prolonged in RSK2-depleted cells, suggesting activation of spindle assembly checkpoint (SAC). Indeed, more BubR1 (Bub1-related kinase) was present on metaphase plate kinetochores in RSK2-depleted cells, and depletion of BubR1 abolished the mitotic accumulation caused by RSK2 depletion, confirming BubR1-dependent SAC activation. Along with the shortening of inter-kinetochore distance, these data suggested that weakening of the tension across sister kinetochores by RSK2 depletion led to the activation of SAC. To test this, we analyzed the RSK2 effects on the stability of kinetochoremicrotubule interactions, and found that RSK2-depleted cells formed less kinetochoremicrotubule fibers. Moreover, RSK2 depletion resulted in the decrease of basal level of microtubule as well as an irregular distribution of mitotic spindles, which might lead to observed several mitotic progression defects such as increase in unaligned chromosomes, defects in chromosome congression and a decrease in pole-to-pole distance in these cells. Taken together, our data reveal that RSK2 affects mitotic progression by regulating the distribution, basal level and the stability of mitotic spindles.
الموضوعات
النص الكامل: 1 الفهرس: WPRIM الموضوع الرئيسي: Phosphotransferases / Protein Kinases / Kinetochores / Ribosomal Protein S6 Kinases / Ribosomal Protein S6 Kinases, 90-kDa / Siblings / M Phase Cell Cycle Checkpoints / Metaphase / Microtubules / Spindle Apparatus المحددات: Humans اللغة: En مجلة: Experimental & Molecular Medicine السنة: 2016 نوع: Article
النص الكامل: 1 الفهرس: WPRIM الموضوع الرئيسي: Phosphotransferases / Protein Kinases / Kinetochores / Ribosomal Protein S6 Kinases / Ribosomal Protein S6 Kinases, 90-kDa / Siblings / M Phase Cell Cycle Checkpoints / Metaphase / Microtubules / Spindle Apparatus المحددات: Humans اللغة: En مجلة: Experimental & Molecular Medicine السنة: 2016 نوع: Article