Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair
Tissue Engineering and Regenerative Medicine
; (6): 605-614, 2019.
Article
ي En
| WPRIM
| ID: wpr-786674
المكتبة المسؤولة:
WPRO
ABSTRACT
BACKGROUND:
In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics.METHODS:
Nasal septum tissue was obtained from five patients aged ≥ 20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages in vitro. To formulate the cell–PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC–PCL complex, and mycoplasma contamination were assessed.RESULTS:
hNCs in PCL showed viability ≥ 70% and remained at these levels for 9 h of incubation at 4 ℃. Immunostaining of the hNC–PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions.CONCLUSION:
The hNC–PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
In Vitro Techniques
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Cartilage
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Cell Survival
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Adhesives
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Fluorescent Antibody Technique
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Collagen
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Chondrocytes
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Collagen Type I
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Tissue Engineering
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Aggrecans
المحددات:
Humans
اللغة:
En
مجلة:
Tissue Engineering and Regenerative Medicine
السنة:
2019
نوع:
Article