The influence of pVR-IL15 gene adjuvant on immunization with recombinant viruses expressing HBsAg / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#To explore the influence of pVR-IL15 gene adjuvant on the immune responses of different immunization strategies.@*Methods@#The sequential immunization strategies were used in BALB/c mice with a DNA vaccine, recombinant modified vaccinia virus Ankara and recombinant adenovirus expressing HBsAg respectively and combined with a gene adjuvant pVR-IL15. Cellular and humoral immune responses were evaluated by IFN-γ enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA), respectively.@*Results@#The levels of humoral and cellular immune responses in the immune groups combined with pVR-IL15 were significantly higher than those of the non-combined with pVR-IL15.@*Conclusions@#The pVR-IL15 gene adjuvant can enhance the immune responses induced by the recombinant viruses expressing HBsAg.