Six new cytotoxic and anti-inflammatory 11, 20-epoxy-ent-kaurane diterpenoids from Isodon wikstroemioides / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 383-389, 2015.
Article
ي En
| WPRIM
| ID: wpr-812531
المكتبة المسؤولة:
WPRO
ABSTRACT
The present study was designed to determine the chemical constituents of EtOAc extracts of the aerial parts of Isodon wikstroemioides. Compounds 1-8 were isolated and purified by normal-phase silica gel and reversed-phase C18silica gel column chromatography and HPLC. Their structures were elucidated by extensive spectroscopic methods. Most of them were evaluated for their in vitro cytotoxicity against human cancer HL-60, SMMC-7721, A-549, MCF-7, and SW-480 cells and their inhibitory activity against nitric oxide (NO) production in LPS-activated RAW264.7 macrophages. Among the eight 11, 20-epoxy-ent-kauranoids isolated, compounds 1-6 (isowikstroemins H-M) were new diterpenoids. Compounds 1, 3, and 7 exhibited significant cytotoxicity with IC50 values ranging from (0.84 ± 0.02) to (4.09 ± 0.34) μmol · L(-1), while compounds 4 and 5 showed selective cytotoxicity. In addition, compounds 1, 3, 4, and 7 exhibited inhibitory activity against nitric oxide (NO) production in LPS-activated RAW264.7 macrophages. These results provide a basis for future development of these compounds as anti-cancer and anti-inflammatory agents.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Plant Extracts
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Chemistry
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Lipopolysaccharides
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Inhibitory Concentration 50
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Isodon
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Plant Components, Aerial
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Cell Line, Tumor
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Diterpenes, Kaurane
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Drug Therapy
المحددات:
Humans
اللغة:
En
مجلة:
Chinese Journal of Natural Medicines (English Ed.)
السنة:
2015
نوع:
Article