Expression of LXR-β in human gastric cancer tissue and the effect of GW3965 on the proliferation of gastric cancer cell line SGC-7901 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 127-133, 2016.
Article
ي Zh
| WPRIM
| ID: wpr-815063
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE@#To examine the expression of liver X receptor-β (LXR-β) in human gastric cancer tissue, and to explore the effect of GW3965, an agonist of LXRs, on proliferation of gastric cancer cell line SGC-7901.
@*METHODS@#The immunohistochemical assay was used to detect the expression of LXR-β, activating transcription factor 4 (ATF4) in gastric cancer tissues and the corresponding pericarcinoma tissues in 114 patients. Real-time quantitative PCR and Western blot were used to determine mRNA and protein levels of ATF4 and ATP-binding cassette 1 (ABCA1), one of the downstream target genes of LXRs, in SGC-7901 cells with or without GW3965 treatment. Cell counting kit-8 (CCK-8) assay was performed to detect cell proliferation. The expression of ATF4 was silenced by short hairpin RNA (shRNA).
@*RESULTS@#The expressions of LXR-β and ATF-4 were obviously down-regulated in the gastric cancer tissues than that in the corresponding pericarcinoma tissues (both P<0.05). Compared with the control cells, GW3965 treatment inhibited proliferation of SGC-7901 cells and up-regulated ATF4 and ABCA1 expressions (both P<0.05). Knockdown of ATF4 can reverse the antiproliferative effect of GW3965 on SGC-7901 cells.
@*CONCLUSION@#The expression of LXR-β is decreased in human gastric cancer tissues, and activation of LXRs by GW3965 could inhibit the proliferation of SGC-7901 cells via ATF4.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pathology
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Pharmacology
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Stomach Neoplasms
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Benzoates
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Benzylamines
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RNA, Messenger
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Gene Expression Regulation, Neoplastic
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Up-Regulation
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Gene Silencing
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RNA, Small Interfering
المحددات:
Humans
اللغة:
Zh
مجلة:
Journal of Central South University(Medical Sciences)
السنة:
2016
نوع:
Article