Optimization of the Extraction Technology of Verbascoside from Cistanche tubulosa by Box-Behnken Response Surface Me- thodology / 中国药房
China Pharmacy
; (12): 1970-1974, 2019.
Article
ي Zh
| WPRIM
| ID: wpr-817216
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE: To optimize the extraction technology of verbascoside from Cistanche tubulosa, and to provide reference for further development and comprehensive utilization of C. tubulosa. METHODS: The content of verbascoside in C. tubulosa was determined by HPLC. The determination was performed on Inertsil-ODS-3V column with mobile phase consisted of methanol-0.2% formic acid aqueous solution (40 ∶ 60, V/V) at the flow rate of 1 mL/min. The column temperature was 30 ℃, the detection wavelength was 330 nm, and the sample size was 10 μL. Using extraction rate of verbascoside as index, soaking time, ethanol concentration, liquid-solid ratio, extraction time and extraction times were investigated by single factor tests. According to the results of above tests, ethanol concentration, liquid-solid ratio and extraction time were optimized by Box-Behnken response surface methodology. The verification tests were carried out on the optimized extraction technology. RESULTS: The linear range of verbascoside was 18.65-932.4 μg/mL. The optimal extraction technology included that ethanol concentration 63%, liquid-solid ratio 8 ∶ 1 (mL/g), soaking for 2 h, extraction time 1.5 h, extracting for 2 times. The extraction rates of verbascoside in the three parallel verification tests were 78.21%, 76.95%, 79.34%, respectively. The relative errors of those to predicted value 76.76% were 1.89%, 0.25%, 3.36%. CONCLUSIONS: The optimized extraction technology of verbascoside from C. tubulosa is stable and feasible, and is suitable for the extraction of verbascoside.
النص الكامل:
1
الفهرس:
WPRIM
نوع الدراسة:
Prognostic_studies
اللغة:
Zh
مجلة:
China Pharmacy
السنة:
2019
نوع:
Article