In vitro study on blocking mTOR signaling pathway in EGFR-TKI resistance NSCLC
Asian Pacific Journal of Tropical Medicine
; (12): 394-397, 2014.
Article
ي En
| WPRIM
| ID: wpr-820698
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect and mechanism of inhibitor everolimus on EGFR-TKI resistance NSCLC.@*METHODS@#MTT assay was used to detect proliferation of human non-small cell lung cancer cell line A549. Flow cytometry was used to detect the changes of apoptosis and cycle distribution in each group after 24 h and 48 h. RT-PCR was used to detect the changes of PTEN and 4EBP1 expression levels after 48 h of monotherapy and combination therapy.@*RESULTS@#MTT assay showed that everolimus had dose-dependent inhibition against growth of A549 cells. Flow cytometry showed when everolimus could induce apoptosis and induce G0/G1 phase cell cycle arrest, which was time-dependent (P<0.05). RT-PCR showed everolimus could increase PTEN and 4EBP1 expression.@*CONCLUSIONS@#mTOR inhibitor everolimus has an inhibitory effect on EGFR-TKI resistant NSCLC, which cannot reverse the resistance effect of EGFR-TKI resistant cell line A549. The relationship between EGFR/AKT signaling pathway and the mTOR signaling pathway and the mechanism in non-small cell lung cancer need further study.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
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Phosphoproteins
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Signal Transduction
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Gene Expression Regulation, Neoplastic
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Apoptosis
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Carcinoma, Non-Small-Cell Lung
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Sirolimus
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Cell Line, Tumor
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Adaptor Proteins, Signal Transducing
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Cell Proliferation
المحددات:
Humans
اللغة:
En
مجلة:
Asian Pacific Journal of Tropical Medicine
السنة:
2014
نوع:
Article