Cloning, expression and characterization of a new endo-β-N-acetylglucosaminidase from Streptomyces alfalfae / 生物工程学报
Chinese Journal of Biotechnology
; (12): 932-941, 2020.
Article
ي Zh
| WPRIM
| ID: wpr-826883
المكتبة المسؤولة:
WPRO
ABSTRACT
Endo-β-N-acetylglucosaminidase is used widely in the glycobiology studies and industries. In this study, a new endo-β-N-acetylglucosaminidase, designated as Endo SA, was cloned from Streptomyces alfalfae ACCC 40021 and expressed in Escherichia coli BL21 (DE3). The purified recombinant Endo SA exhibited the maximum activity at 35 ºC and pH 6.0, good thermo/pH stability and high specific activity (1.0×10⁶ U/mg). It displayed deglycosylation activity towards different protein substrates. These good properties make EndoSA a potential tool enzyme and industrial biocatalyst.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Streptomyces
/
Enzyme Stability
/
Recombinant Proteins
/
Gene Expression
/
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
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Cloning, Molecular
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Escherichia coli
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Genetics
/
Metabolism
اللغة:
Zh
مجلة:
Chinese Journal of Biotechnology
السنة:
2020
نوع:
Article