Effect of Chidamide on the Killing Acitivity of NK Cells Targeting K562 Cells and Its Related Mechanism In Vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1167-1170, 2020.
Article
ي Zh
| WPRIM
| ID: wpr-827145
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of chidamide on the killing activity of NK (Natural killer cell, NK) cells targeting K562 cells and its related mechanism.@*METHODS@#K562 cells were pretreated with chidamide at different concentrations and cocultured with NK cells at different effect-target ratios. The killing effect of chidamide on K562 cells by NK cells, the expression of natural killer group 2 member D (NKG2D) ligands and apoptosis rate of K562 cells were detected by flow cytometry.@*RESULTS@#The killing sensitivity of NK cells to K562 cells could be enhanced by chidamide. The expression of ULBP2 on K562 cell surface could be up-regulate, however, the expression of ULBP1 and MICA/MICB showed no statistically difference as compared with control group. Chidamide showed no obvious cytotoxicity to K562 cells.@*CONCLUSION@#Chidamide can significantly improve killing efficiency of NK cells on K562 cells, which may be related to the up-regulation of ULBP2 expression.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Benzamides
/
Killer Cells, Natural
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Histocompatibility Antigens Class I
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K562 Cells
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Intercellular Signaling Peptides and Proteins
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Allergy and Immunology
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NK Cell Lectin-Like Receptor Subfamily K
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GPI-Linked Proteins
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Aminopyridines
المحددات:
Humans
اللغة:
Zh
مجلة:
Journal of Experimental Hematology
السنة:
2020
نوع:
Article