Comparison of surface markers of mesenchymal stem cells from different sources / 解剖学报

ABSTRACT

Objective To investigate the expression difference of surface markers of human umbilical cord mesenchymal stem cells (HUCMSCs), human adipose mesenchymal stem cells (ADSCs) and human fetal blood source endometrial mesenchymal stem cells (MenSCs) and the changes of surface markers with the culture generation. Methods HUCMSCs, ADSCs and MenSCs were cultured to passage 3, 6, 9 and 12. Five MSC-specific markers, CD29, CD44, CD73, CD90 and CD 105, and one HSC markers, CD45, were assessed by flow cytometry and immunofluorescence. Results The cultured MenSCs and HUCMSCs were spindle-shaped, and the ADSCs forms were mainly spindle-shaped and multiple-angular. The results of flow cytometry showed that the MSC-positive markers including CD29, CD44, CD73 and CD105 were highly expressed by at least 95% in the passage 3 HUCMSCs, ADSCs and MenSCs cells and CD45 negative expression. Specifically, CD90 levels of MenSCs of passage 3 was (72. 43 ± 0. 7 6) %, which was lower than that in HUCMSCs (99.67±0. 12)% and ADSCs (99. 70 ± 0. 15)% (P 0. 05). The results of immunofluorescence was consistent with those of flow cytometry. Conclusion With the increase of culture time, HUCMSCs, ADSCs and MenSCs are stable in high expression of CD29, CD44, CD73, CD90 and CD 105, which does not express CD45, while the CD90 expression rate of MenSCs is lower than that in HUCMSCs and ADSCs.