Study on fingerprint of Aucklandiae Radix based on principal component analysis coupled with cluster analysis / 中草药

ABSTRACT

Objective: To establish a UPLC fingerprint method and a method for the content determination of 3,5-O-dicaffeoylquinic acid of Aucklandiae Radix, and provide a comprehensive evaluation of the drug from different habitats. Methods: UPLC analysis was performed on a YMC Trait C18 (100 mm × 2.1 mm, 1.9 μm), with a mobile phase consisting of acetonitrile-0.05% phosphoric acid at the flow rate of 0.30 mL/min. The fingerprint detection wavelength was 254 nm and the content determination detection wavelength of 3,5-O-dicaffeoylquinic acid was 327 nm, meanwhile, the column temperature was controlled at 30 ℃. Similarity analysis, hierarchical clustering analysis, and principal component analysis were undertaken to investigate the fingerprints of 13 batches of Aucklandiae Radix. Results: UPLC fingerprint of Aucklandiae Radix was established and eight common peaks were designated. The results showed that the quality of the batches of samples were not stable. Samples collected from the same region and different regions both had certain differences, as well as the content determination of 3,5-O-dicaffeoylquinic acid. Conclusion: The proposed method offered a fast, holistic, and effective method for the quality control of Aucklandiae Radix.