Simultaneous determination of eleven constituents in Yigan Yiqi Jieyu Granules by HPLC / 中草药
Chinese Traditional and Herbal Drugs
; (24): 4346-4351, 2019.
Article
ي Zh
| WPRIM
| ID: wpr-850844
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective: To establish HPLC method for the simultaneous determination of saikosaponin a, naringin, paeoniflorin, calycosin-7-glucoside, tanshinone IIA, cinnamaldehyde, schisandrin, syringin, berberine hydrochloride, chrysophanol and hesperidin in Yigan Yiqi Jieyu Granules (YYJG), and conduct a quality assessment using principal component analysis. Methods: The chromatographic separation was achieved on an Caprisil AQ-C18 (150 mm × 4.6 mm, 5.0 μm) column with mobile phase consisted of 0.1% phosphate-acetonitrile for gradient elution, at the flow rate of 0.8 mL/min; The column temperature was 45 ℃. The results of the content were then combined with the principal component analysis to achieve the scientific assessment of the different batches of drugs. Results: The content of saikosaponina, naringin, paeoniflorin, calycosin-7-glucoside, tanshinone IIA, cinnamaldehyde, schisandrin, syringin, berberine hydrochloride, chrysophanol and hesperidin in YYJG had good linear relationship in the ranges of 1.6-80.0, 14-700, 10-500, 1.6-80.0, 1.6-80.0, 2.4-120.0, 1.2-60.0, 1.2-60.0, 8.0-400.0, 2.0-100.0, and 2.0-100.0 μg/mL, respectively; The average sample recovery rate range were 98.3%, 99.2%, 98.8%, 99.3%, 101.9%, 97.5%, 99.8%, 101.7%, 101.1%, 102.5%, and 100.9% (RSD < 2.0%); The content of 11 active ingredients in 16 batches of samples respectively were 0.233-0.322, 3.007-3.142, 2.201-2.273, 0.320-0.355, 0.317-0.399, 0.451-0.523, 0.265-0.297, 0.209-0.226, 1.848-1.873, 0.380-0.425, and 0.615-0.647 mg/g, respectively. Conclusion: The established method is simple, accurate and reproducible, and can provide the reference for the quality control of YYJG.
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
Zh
مجلة:
Chinese Traditional and Herbal Drugs
السنة:
2019
نوع:
Article