Cloning and bioinformatic analysis of mevalonate diphosphate decarboxylase gene promoter in Eleutherococcus senticosus / 中草药

ABSTRACT

Objective: To clone and analyze the sequence of mevalonate diphosphate decarboxylase (MDD) gene promoter from Eleutherococcus senticosus. Methods: According to the full length of MDD cDNA sequence, using PCR cloning and TAIL-PCR methods, we are cloning 5'DNA sequence and promoter sequence of MDD gene. And bioinformatic analysis is used. Such as PlantCARE. Results: We have cloned the 5'DNA sequence successfully, with full length of 1024 bp. And the promoter sequence is 1423 bp. Bioinformatic analysis shows that the promoter sequence has 49 TATA-box and 25 CAAT-box. In addition, the promoter has some cis-acting elements responsive to abscisic acid, environmental stresses, MeJA, required for endosperm expression, light-regulated and so on. Last but not least, there have two MYBHv1 and two MYB binding sites. Conclusion: We have firstly cloned and analyzed the E. senticosus MDD gene promoter sequence. It is important to it's expression.