Regulation and mechanism of corylin on inflammasomes / 中国药理学通报

ABSTRACT

Aim: To investigate the regulation effect and molecular mechanism of corylin on NLRP3, NL-RC4, AIM2 inflammasomes with immortalized bone marrow-derived macrophages. Methods: The effect of corylin on the NLRP3, NLRC4, AIM2 inflammasome activation was evaluated with LPS-induced ATP, nigericin, salmonella, poly(dA dT). Caspase-1 activity was determined by Caspase-Glo® 1 Inflammasome Assay. Western blot was performed to observe the protein expression levels of mature IL-1β, caspase-1 p20 in the culture supernatants, pro-caspase-1, pro-IL-1β, ASC, NLRP3 in the cell lysates. Results: Corylin blocked the self-slicing of pro-caspase-1 induced by ATP, nigericin, salmonella and poly(dA dT), then suppressed the secretion of mature IL-1β mediated by caspase-1, which showed that corylin inhibited the NL-RP3, NLRC4, AIM2 inflammasomes activation. Moreover, corylin irreversibly attenuated the activation of NLRP3 inflammasome without affecting NF-κB signaling pathway. Conclusions: Corylin inhibits the inflammasome activation of NLRP3, NLRC4, and AIM2, and further reduces its mediated immune inflammatory response. Meanwhile, it provides new ideas and strategies for the treatment of immune inflammatory diseases by using corylin-related preparations.