Synergistic Effect of Baicalin and Wogonoside on NF-κB Signaling Pathway Based on Medium-efficiency Equation / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
; (24): 84-91, 2020.
Article
ي Zh
| WPRIM
| ID: wpr-872763
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective:
Quantitative analysis of anti-inflammatory synergistic pharmacodynamics mechanism of baicalin and wogonoside by medium efficiency principle.Method:
inflammatory cell model was constructed by stimulating RAW264.7 cells by lipopolysaccharide (LPS) 100 μg·L-1 in vitro. The experiment was performed in the normal group, the model group, the andrographolide group (10 μmol·L-1), the baicalin group (2.06,4.13,8.25,16.5,33,66,132 μmol·L-1) and the wogonoside group (2.94,5.88,11.75,23.5,47,94,188 μmol·L-1) and the baicalin-wogonoside combination group [(2.06+2.94)(4.13+5.88)(8.25+11.75)(16.5+23.5)(33+47)(66+94)(132+188) μmol·L-1]. The levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the cell culture supernatants after drug intervention for 50 min and 4 h were detected by enzyme-linked immunosorbent assay (ELISA) method. The level of nitric oxide (NO) in the cell culture supernatant after drug intervention for 24 h were detected by Griess method. Western blot was used to detect the activation levels of phosphorylation of nuclear factor-κB p65(p-NF-κB p65) and inducible nitric oxide synthase(iNOS) in cells after drug intervention for 2 h and 12 h. The fa/fu-dose profile of each indicator was drawn to observe the increase or decrease of effect.Result:
Compared with normal group, the expression of p-NF-кB p65, iNOS and cytokines including TNF-α, IL-6 and NO (P<0.05,P<0.01) in the model group were significantly up-regulated. Compared with the model group, each group at high doses could inhibit the phosphorylation of NF-кB p65 protein(P<0.05),the baicalin group and the combined group could down-regulate the expression of iNOS protein in a concentration-dependent manner(P<0.01) and the baicalin group had no obvious inhibitory effect. each administration group at high dose could significantly inhibit the production of NO(P<0.05),but each group had no inhibitory effect on IL-6 production. The baicalin group and the combined group could significantly Inhibit the production of TNF-α(P<0.05) and there was no significant difference between the baicalin group and the model group. At the experimental dose, the fa/fu-dose table showed that the fa/fu value of p-NF-кB p65 and IL-6 in the combined group was not greater than the baicalin group and the wogonoside group. The fa/fu value of iNOS, TNF-α and NO in the combined group is higher than the baicalin group and the wogonoside group.Conclusion:
The baicalin and wogonoside have different effects on different targets in the NF-κB pathway. The wogonoside is the main pharmacological substance in this combination and the combination shows different degrees of synergy or antagonism effects on different targets.
النص الكامل:
1
الفهرس:
WPRIM
نوع الدراسة:
Prognostic_studies
اللغة:
Zh
مجلة:
Chinese Journal of Experimental Traditional Medical Formulae
السنة:
2020
نوع:
Article