Rapid screening of single guide RNA targeting pig genome and the harvesting of monoclonal cells by microarray seal / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 111-121, 2021.
Article
ي Zh
| WPRIM
| ID: wpr-879256
المكتبة المسؤولة:
WPRO
ABSTRACT
The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Swine
/
Clustered Regularly Interspaced Short Palindromic Repeats
/
CRISPR-Cas Systems
/
Gene Editing
نوع الدراسة:
Diagnostic_studies
/
Screening_studies
المحددات:
Animals
اللغة:
Zh
مجلة:
Journal of Biomedical Engineering
السنة:
2021
نوع:
Article