Toll-like receptor 9 dependent activation of MAPK and NF-kB is required for the CpG ODN-induced matrix metalloproteinase-9 expression
Experimental & Molecular Medicine
; : 239-245, 2007.
Article
ي En
| WPRIM
| ID: wpr-90608
المكتبة المسؤولة:
WPRO
ABSTRACT
Unmethylated CpG oligodeoxynucleotides (CpG ODNs) activate immune cells to produce immune mediators. This study demonstrates that in murine macrophage RAW 264.7 cells, CpG ODN-mediated matrix metalloproteinase-9 (MMP-9) expression is regulated at transcriptional level and requires de novo protein synthesis. Inhibition of ERK and p38 MAPK, but not JNK, results in significant decrease of CpG ODN-induced MMP-9 expression. We found that endosomal maturation inhibitors, chloroquine and bafilomycin A, block CpG ODN-induced ERK and p38 MAPK activation and the subsequent MMP-9 expression. We also observed that CpG ODN induces NF-kappa B activation and NF-kappa B is a downstream target of p38 MAPK. Taken together, our data demonstrate that CpG ODN triggers MMP-9 expression via TLR-9 dependent ERK and p38 MAPK activation followed by NF-kappa B activation.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Oligodeoxyribonucleotides
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Signal Transduction
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Cell Line
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Enzyme Induction
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NF-kappa B
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Mitogen-Activated Protein Kinase 1
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Matrix Metalloproteinase 9
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Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinase 3
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P38 Mitogen-Activated Protein Kinases
المحددات:
Animals
اللغة:
En
مجلة:
Experimental & Molecular Medicine
السنة:
2007
نوع:
Article