A comparative study of microwire electrode array with built-in and external reference electrodes / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 85-90, 2022.
Article
ي Zh
| WPRIM
| ID: wpr-927903
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective:
To compare the difference between the built-in and external reference electrode of microwire electrode array in the process of recording rat brain neuron firings, optimizing the production and embedding of the microwire electrode array, and providing a more affordable and excellent media tool for multi-channel electrophysiological real-time recording system.Methods:
A 16 channel microwire electrode array was made by using nickel chromium alloy wires, circuit board, electrode pin and ground wires (silver wires). The reference electrode of the microwire electrode array was built-in (the reference electrode and electrode array were arranged in parallel) or external (the reference electrode and ground wire were welded at both ends of one side of the electrode), and the difference between the two electrodes was observed and compared in recording neuronal discharges in ACC brain area of rats. Experimental rats were divided into built-in group and external group, n=8-9. The test indicators included signal-to-noise ratio (n=8), discharge amplitude (n=380) and discharge frequency (n=54).Results:
The microwire electrode array with both built-in and external reference electrodes successfully recorded the electrical signals of neurons in the ACC brain region of rats. Compared with the external group, the electrical signals of neurons in built-in group had the advantages of a higher signal-to-noise ratio (P<0.05), a smaller amplitude of background signals and less noise interference, and a larger discharge amplitude(P<0.05); there was no significant difference in spike discharge frequency recorded by these two types of electrodes (P>0.05).Conclusion:
When recording the electrical activity of neurons in the ACC brain region of rats, the microwire electrode array with built-in reference electrode recorded electrical signals with higher signal-to-noise ratio and larger discharge amplitude, providing a more reliable tool for multi-channel electrophysiology technology.Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Brain
/
Action Potentials
/
Electrophysiological Phenomena
/
Microelectrodes
/
Neurons
المحددات:
Animals
اللغة:
Zh
مجلة:
Chinese Journal of Applied Physiology
السنة:
2022
نوع:
Article