Application of oral fluid in SARS-CoV-2 nucleic acid and antibody detection / 中华预防医学杂志
Zhonghua Yu Fang Yi Xue Za Zhi
; (12): 355-359, 2022.
Article
ي Zh
| WPRIM
| ID: wpr-929578
المكتبة المسؤولة:
WPRO
ABSTRACT
This study aimed to explore the application value of new biological specimen oral fluid in SARS-CoV-2 nucleic acid and antibody detection. Oral fluid and paired respiratory and blood specimens from 7 confirmed cases of two COVID-19 cluster epidemic were collected in Beijing from October to November 2021. SARS-CoV-2 virus and IgG antibody were detected by real time PCR kits and serum antibody detection reagents, and SARS-CoV-2 IgG antibody in oral fluids was detected by a new established method of magnetic particle chemiluminescence. The results showed that the nucleic acid amplification test of SARS-CoV-2 on nasopharyngeal swabs, throat swabs and oral fluid specimens from 3 confirmed cases of COVID-19 was positive, among which the Ct value for ORF1a/b and N gene of oral fluid samples in 2 cases was close to that of throat swab, and the Ct value of oral fluid sample for 1 case was higher than that of throat swab. The complete genome sequence of one oral fluid specimen was obtained, which belonged to the VOC/Delta variant strain. The SARS-CoV-2 IgG antibodies of the paired oral fluid and serum were all positive, and the S/CO values of oral fluid were all lower than those of serum. The series of oral fluid results showed that SARS-CoV-2 IgG antibody level increased from 11 to 32 days after the onset of the disease.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Nucleic Acids
/
Sensitivity and Specificity
/
SARS-CoV-2
/
COVID-19
نوع الدراسة:
Diagnostic_studies
المحددات:
Humans
اللغة:
Zh
مجلة:
Zhonghua Yu Fang Yi Xue Za Zhi
السنة:
2022
نوع:
Article