Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method
Asian Pacific Journal of Tropical Biomedicine
; (12): 281-292, 2020.
Article
ي Zh
| WPRIM
| ID: wpr-950298
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective: To optimize the ultrasonication method for efficient extraction of P-sitosterol and lupeol from the roots of Astragalus atropilosus using Box-Behnken design of response surface methodology (RSM), and its validation by high performance thin layer chromatography (HPTLC) method. Methods: Ultrasonication method was used to extract β-sitosterol and lupeol from Astragalus atropilosus (roots). RSM was used to optimize the different extraction parameters viz. liquid to solid ratio (10-14 mL/g), temperature (60-80 °C) and time (40-60 min) to maximize the yield of β-sitosterol and lupeol. The quantitative estimation of β-sitosterol and lupeol was done in chloroform extract of Astragalus atropilosus by validated HPTLC method on 10 cm × 20 cm glass-backed silica gel 60F
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
Zh
مجلة:
Asian Pacific Journal of Tropical Biomedicine
السنة:
2020
نوع:
Article