Preparation and identification of rabbit anti-mouse coiled-coil domain containing 189(Ccdc189)polyclonal antibody / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
; (12): 456-462, 2023.
Article
ي Zh
| WPRIM
| ID: wpr-981886
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective To prepare a rabbit anti-mouse coiled-coil domain containing 189 (Ccdc189) polyclonal antibody. Methods The pET-28a-Ccdc189 prokaryotic expression plasmid was constructed and transformed into E.coli BL21. IPTG was used to induce the expression of Ccdc189 prokaryotic protein. Adult male New Zealand rabbits were immunized with purified recombinant protein to obtain rabbit anti-mouse Ccdc189 polyclonal antibody. The specificity of the polyclonal antibody was identified by Western blot analysis, indirect ELISA and immunofluorescence histochemical staining. Results The pET-28a-Ccdc189 recombinant plasmid was successfully constructed and the expression of the Ccdc189 recombinant protein was induced. ELISA revealed that the titer of the polyclonal antibody was 11 000 000. Western blot and immunofluorescence staining demonstrated that the Ccdc189 polyclonal antibody could specifically identify the Ccdc189 prokaryotic protein and the Ccdc189 protein in adult wild-type mouse testis. Conclusion A polyclonal antibody with high specificity against mouse Ccdc189 was successfully created.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Recombinant Proteins
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Enzyme-Linked Immunosorbent Assay
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Blotting, Western
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Escherichia coli
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Antibodies
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Antibody Specificity
المحددات:
Animals
اللغة:
Zh
مجلة:
Chinese Journal of Cellular and Molecular Immunology
السنة:
2023
نوع:
Article