The expression of FN-IIIcs in the wound healing and its relationship with injury time / 法医学杂志

ABSTRACT

OBJECTIVE@#To research the expression of alternative splicing segment of fibronectin-IIIcs in incised wound of skin, to offer the experimental data for early injury time judgement.@*METHODS@#Using in situ hybridization with DIG-labeled anti-sense RNA probe, the expression of FN-IIIcs domain was detected in both human and rat skin incised wound.@*RESULTS@#(1) The expression of FN-IIIcs improved from the time of 30 min after injury, reached peak at 6 h and then reduced gradually. (2) The positive expression cells were mainly distributed in hair follicles, sebaceous glands and endothelium, however, in human beings they were basement cells of epidermis.@*CONCLUSION@#The expression of FN-IIIcs domain would be a supposed useful criteria for early injury time judgement.